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KMID : 1144820220280030200
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대한의생명과학회지
2022 Volume.28 No. 3 p.200 ~ p.205
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Comparison of CXCL10 Secretion in Colorectal Cancer Cell Lines
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Lee Song-Mi
Lee Ji-Eun
Ahn Hye-Rim
Choi Myung-Hyun
Yoon Seo-Young
Rhee Man-Hee
Baik Ji-Sue
Seo You-Na
Park Moon-Taek
Kim Sung-Dae
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Abstract
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Established cancer cell lines are widely used for developing biomarkers for the patient-specific treatment of colorectal cancer and predicting prognoses. However, cancer cell lines may exhibit different drug responses depending upon the characteristics of the cell line. Therefore, it is necessary to select a tumor cell line suitable for the purpose of the study by considering the cell characteristics. This study investigated the levels of CXCL10, which were recently been reported to play an important role in the outcome of tumor treatment, secreted by colon cancer cells. 2 × 105 cells/mL of each colorectal cancer cell was seeded into a 35 mm cell culture dish. After 24 h incubation, culture supernatant was used to determine the secreted CXCL10 levels. Among six colorectal cancer cell lines (HT-29, HCT116, CaCo-2, SW620, SW480, and CT26), Caco-2 cells showed the highest level of CXCL10 secretion. HT-29 cells showed the second-highest level of CXCL10 secretion. No significantly measurable level of CXCL10 secretion was detected in HCT116 cells. These results will be helpful in investigating the molecular basis of colorectal cancer.
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KEYWORD
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Colorectal cancer, CXCL10, Chemotherapy, Chemokine, Biomarker
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